TABLE 2.
S. cerevisiae strains and plasmids
| Name | Relevant characteristics | Ref./Source |
|---|---|---|
| S. cerevisiae strains | ||
| Wild-type (SEY6210) | MATα trp-Δ901 leu2-3, 112 his3-Δ200 ura3-52 lys2-801 suc2-Δ9 can1::hisG | Mayinger and co-workers (81) |
| Δinp54/Δsac1 | MATα trp-Δ901 leu2–3, 112 his3- Δ200 ura3–52 lys2–801 suc2-Δ9 can1::hisG sac1::TRP1, inp54::LEU2 | Mayinger and co-workers (61) |
| S. cerevisiae plasmids | ||
| pYC2/NT A | Glu-repressible, Gal-inducible yeast expression vector | Invitrogen |
| pYC-ExoUP.aer-S142A-GFP | For expression of ExoUP.aer-S142A-GFP | This study |
| pYC-ExoUP.flu-S92A-GFP | For expression of ExoUP.flu-S92A-GFP | This study |
| pYC-ExoUP.asy-S137A-GFP | For expression of ExoUP.asy-S137A-GFP | This study |
| pYC-ExoUP.asy MLD-GFP | For expression of ExoUP.asy MLD-GFP | This study |
| pYC-2x(GFP-PH) | For expression of GFP-tagged pleckstrin homology domain from PLCδ | Orth and co-workers (72) |