TABLE 3.

Primers used in this study

Name	Sequence (5′ → 3′)a
For purification
5′ ExoUP.aer MLD HindIII	AAAAAGCTTATCACAGACGGGGCGGTG
3′ ExoUP.aer MLD NotI	AAAGCGGCCGCCTGTGAACTCCTTATTCCG
5′ ExoUP.flu HindIII	AAAAAGCTTATGAAAGTCTCCAGTTCT
3′ ExoUP.flu NotI	AAAGCGGCCGCCAACCGGAATACGCCAGGC
5′ ExoUP.flu MLD HindIII	AAAAAGCTTGCCGGCGCCGAGGCGTTG
5′ ExoUP.asy HindIII	AAAAAGCTTATGCAAATTCACATTAAT
3′ ExoUP.asy NotI	AAAGCGGCCGCCTGCTGTTTTGATCATCCA
5′ ExoUP.asy MLD HindIII	AAAAAGCTTTCAACCTCAAACATTGCT
For transfection
5′ ExoUP.flu AgeI	AAAACCGGTGATGAAAGTCTCCAGTTCT
3′ ExoUP.flu NotI	AAAGCGGCCGCCCTAAACCGGAATACGCCA
5′ ExoUP.asy AgeI	AAAACCGGTGATGCAAATTCACATTAAT
3′ ExoUP.asy NotI	AAAGCGGCCGCCCTATGCTGTTTTGATCAT
5′ ExoUP.aer MLD AgeI	AAAACCGGTGATCACAGACGGGGCGGTG
3′ ExoUP.aer NotI	AAAGCGGCCGCCTCATGTGAACTCCTTATT
5′ ExoUP.flu MLD AgeI	AAAACCGGTGGCCGGCGCCGAGGCGTTG
5′ ExoUP.asy MLD AgeI	AAAACCGGTGTCAACCTCAAACATTGCT
For site-directed mutagenesis
5′ ExoUP.flu S92A	GTGGTTTCCGGCTCAGCGGCCGGTGCGATTTG
3′ ExoUP.flu S92A	CAAATCGCACCGGCCGCTGAGCCGGAAACCAC
5′ ExoUP.flu R616L	GTCAAACATTACCGAGCGCTCAATAAACCCTGGAGTAAAC
3′ ExoUP.flu R616L	GTTTACTCCAGGGTTTATTGAGCGCTCGGTAATGTTTGAC
5′ ExoUP.asy S137A	CACCATGTCAGGTTCTGCTGCGGGCGGGATCAC
3′ ExoUP.asy S137A	GTGATCCCGCCCGCAGCAGAACCTGACATGGTG
5′ ExoUP.asy R652L	GGCGGACAATTATGGCGCACTAATGAAACCTTGGAAAAAAC
3′ ExoUP.asy R652L	GTTTTTTCCAAGGTTTCATTAGTGCGCCATAATTGTCCGCC
For yeast cloning
5′ GFP yeast	GACTCACTATAGGGAATATTAAGCTTACCATGGCCAGCAAAGGAGAAGAA
3′ ExoUP.aer	GATAGGCTTACCTTCGAAGGGCCCTCTAGATCATGTGAACTCCTTATTCC
3′ ExoUP.flu	GATAGGCTTACCTTCGAAGGGCCCTCTAGACTAAACCGGAATACGCCAGG
3′ ExoUP.asy	GATAGGCTTACCTTCGAAGGGCCCTCTAGACTATGCTGTTTTGATCATCC

^a Restriction sites are in bold.