FIGURE 6.

Aggregation kinetics of α-syn in the presence of SUVs. Aggregation kinetics for α-syn was measured by ThT fluorescence in the presence of SUVs in 10 mm MES, pH 5.5, with 140 mm NaCl. The averages of eight replicate traces are shown in bold with individual traces dashed below. A, aggregation of 30 μm α-syn (black) with 0.2 mm DOPC SUVs (red). B, aggregation of 30 μm α-syn (black) with 0.2 mm 30% DOPS SUVs (green) or 0.2 mm 6% cardiolipin SUVs (blue). C, aggregation of 30 μm α-syn (black) with 0.2 mm 15% sphingosine SUVs (orange). All mixtures are based on DOPC with addition of the indicated lipid. In all instances, the aggregation is retarded or shows no significant change in lag time when the lipid vesicles are added. D, aggregation of 30 μm α-syn alone (black) with extracted exosome lipid SUVs (green). Addition of SUVs made from extracted exosome lipids was the only model system studied that catalyzed the aggregation kinetics of α-syn.