FIGURE 6.

Notch mutants partially rescue the cartilage phenotypes in MLII zebrafish. A, representative Alcian blue stains of WT, MLII, and rescued embryos. The percentage of embryos with the representative staining pattern is shown. B, schematic representation of four different parameters, including the angle, length, and position of multiple cartilage elements, quantified for all embryos in each condition. PF, pectoral fin; CB, cartilage. C, measurements were made in at least 25 embryos for each condition. The results of this quantification support partial rescue when Notch mutant mRNA is used; see “Results” for details. All parameters evaluated in the MLII zebrafish were significantly altered compared with WT zebrafish (p < 0.001). The parameters in the rescued zebrafish (WT, C447Y, and C473S) were all significantly improved compared with the MLII zebrafish (p < 0.01). PQ, palatoquadrate.