FIGURE 2.

The NUT protein recruits p300 to stimulate local histone H4 acetylation and BRD4 enrichment in the transgene locus. U2OS 2-6-3 cells were transfected with LacI-CHERRY-BRD4S or LacI-CHERRY-NUT. 24 h post-transfection, cells were immunostained with p300, CBP, AcH4, or BRD4 C antibodies and counterstained with DAPI. BRD4 C antibody is specific for the BRD4 long isoform. IF, immunofluorescent staining with indicated antibodies (p300, CBP, AcH4, or BRD4). The RGB intensity profiles show the intensity curve of red, green, and blue signals along the highlighted yellow bars in the Merge panel. Scale bars = 5 μm.