FIGURE 6.

Visualization of the tighter BRD4 chromatin binding induced by the NUT protein tethered to chromatin. U2OS cells were transfected with vector control (V) or constructs for expressing Xpress-tagged BRD4S, BRD4-NUT, or H2B-NUT. 36 h post-transfection, coverslips were collected and either fixed immediately (Non-treated) or pre-extracted in a buffer containing 250 mm NaCl prior to fixation (Salt-treated). The cells were then stained with Xpress (green) and Brd4 C (red) antibodies and counterstained with DAPI. Representative images from three experiments are presented. Scale bars = 10 μm.