TABLE 1.
RT-PCR primers used to detect transcripts for gel-forming mucins in human submandibular glands
The positions of primers are based on reference sequences (GenBankTM accession numbers). PCRs incorporated 1 unit of AccuprimeTM Taq high fidelity DNA polymerase, 0.25 μm primers, and 20 ng of cDNA. Cycling conditions were as follows: 5 min at 94 °C; 35 cycles of 1 min at 94 °C, 1 min at 55 °C, and 2 min at 68 °C followed by 7 min at 68 °C.
| Transcript | Reference sequence | Primer sequence | Exons | Amp |
|---|---|---|---|---|
| bp | ||||
| MUC2 | NM_002457 | 5′-CAAGCACAGCACCGATTGCTGAGT-3′ | 22 | 441 |
| 5′-CACCTGGTGCGTAGTAGGTGTCGTT-3′ | 23 | |||
| MUC5AC | AJ001402 | 5′-GTTCTCCGGCCTCATCTTCTCC-3′ | 5 | 350 |
| 5′-GCTCAAAGACCTTGCTCAGAATCAG-3′ | 7 | |||
| MUC5B | NM_002458 | 5′-CAGGAGTCCATCTGCACCCAG-3′ | 42 | 294 |
| 5′-CCAGGTTTCATTCAGCTGGAC-3′ | 45/46 | |||
| MUC6 | U97698 | 5′-AGGAGGAGATCACGTTCAAG-3′ | 32 | 303 |
| 5′-TGTCATCTGCAGTCCTTCAG-3′ | 33 | |||
| MUC19 | AY236870 | 5′-GAGTGCCAACAAGACCACAAG-3′ | 26 | 820 |
| 5′-GGCCTGTTAAAACTGGGAAGG-3′ | 35 |