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. 2014 Dec 17;290(5):2784–2797. doi: 10.1074/jbc.M114.593384

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FIGURE 3. — Htra1 transcription regulator activity and Htra1 protein expression in the mouse retina. A, immunohistochemistry to detect HtrA1 expression in HtrA1^+/+, HtrA1^+/−, HtrA1^−/−, and HtrA1-Tg mouse retinas (blue, DAPI; green, HtrA1). NC (mouse IgG), staining control; scale bars, 100 μm. B, immunohistochemistry to detect HtrA1 expression in HtrA1^+/− mouse retinas (red, β-galactosidase; green: HtrA1; blue, DAPI; yellow, merge). Scale bars, 100 μm. C, in situ hybridization to detect HtrA1 expression in HtrA1^+/− mouse retina tissues (red arrowheads, HtrA1; blue arrowheads, β-galactosidase). Scale bars, 100 μm. OS, outer segment; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.