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. 2014 Dec 10;290(5):3172–3182. doi: 10.1074/jbc.M114.619890

FIGURE 4.

FIGURE 4.

PLP2 inhibits the p53-dependent apoptosis. A, PLP2 expression decreases the steady-state level of Puma. p53−/− MEF cells were transfected with Flag-p53 and increasing amounts of V5-PLP2. After 48 h, total lysates were immunoblotted to detect the expression of Puma and p53. B, poly(I:C) increases the transcription of the P53 gene. p53 mRNA levels were analyzed by qPCR. The data are shown as the means ± S.D. (n = 3). C and D, overexpression of PLP2 decreases p53-dependent apoptosis. Apoptosis in p53+/+ and p53−/− HCT116 cells was determined by staining with annexin V. The data are shown as the means ± S.D. (n = 3). The differences are statistically significant (**, p value < 0.001). NS, not significant (p value > 0.05).