FIGURE 7.

iPLA₂γ reduced tunicamycin-induced cell death. GECs were transfected with GFP-iPLA₂γ or empty vector in control. After 24 h, cells were untreated or were treated with tunicamycin (Tm, 1, 5, or 10 μg/ml) for 24 h. Tunicamycin-induced cytotoxicity was monitored by LDH release. The results show Tm-induced LDH release above the values in untreated cells. Tunicamycin (5 and 10 μg/ml) significantly induced LDH release in vector-transfected cells. This effect was reduced in GECs overexpressing iPLA₂γ. *, p < 0.05; **, p < 0.01 Tm versus untreated (Vector). +, p < 0.01 iPLA₂γ versus vector (Tm 10), five experiments performed in duplicate.