Signal changes
induced by antibiotic treatments in E. coli with
different susceptibilities. All data were collected in the
presence of MH-IR786. (A to F) Scatter signal changes. The pseudocolor
plots are the no antibiotic paired control, for each strain. The overlaid
contour plots are the 1x MIC antibiotic concentration scatter data.
(A to C) The lab strain E. coli (ATCC 33456). (D
to F) The multidrug clinical strain E. coli (Mu14S).
(G to I) PB-sQF 3D test results. First column (A, D, and G) penicillin
G; second column (B, E, and H) tetracycline; third column (C, F, and
I) gentamicin. Penicillin G and tetracycline were examined at the
1x, 1/4x, and 1/16x of MIC of ATCC (32 and 1 μg/mL, respectively).
Gentamicin was applied at the MIC of Mu14S (4 μg/mL). FSC: forward
scatter. SSC: side scatter. (Corresponding fluorescence data can be
found in the Supporting Information).