Table III.

Recommendations on antibody titration.

Rec. n.	Recommendation	GoR
10	In order to perform anti-D titration, it is suggested that D-heterozygous (R1r, R2r) red blood cells are used, because these are best able to demonstrate foetal antigen expression. In any case, the same test red blood cells with the same zygosity must always be used in order to be able to interpret the trend in titres correctly.	2B
11	If a pregnant woman has anti-D antibodies, or other antibodies that are clinically significant with regards to HDFN, it is suggested that the antibody titre is measured every 4 weeks until the 18th week of gestation and then every 2–4 weeks. The results of the titration must be compared with those obtained in the previously tested samples, using test red blood cells with the same antigen expression.	2C