Figure 7.

Colocalization of RHBG (brown) with H⁺-ATPase (blue) in the epididymis. In order to determine specific epididymal cell types expressing RHBG, we colocalized RHBG with H⁺-ATPase. In the initial segment, basolateral RHBG immunoreactivity (brown) is present in H⁺-ATPase-positive narrow cells (arrows) and H⁺-ATPase-negative principal cells (arrowheads). In the caput epididymidis, basolateral RHBG immunoreactivity is present exclusively in H⁺-ATPase-positive clear cells (arrows). In the upper corpus, both H⁺-ATPase-positive clear cells (arrows) and a subset of H⁺-ATPase-negative principal cells (arrowheads) expressed basolateral RHBG immunolabel. In the middle and lower corpus and cauda epididymidis, H⁺-ATPase-positive clear cells (arrows), but not H⁺-ATPase-negative principal cells, expressed basolateral RHBG immunolabel. In the vas deferens, basolateral RHBG was present only in H⁺-ATPase-positive cells (arrows). In all regions, intraluminal sperm did not label for either RHBG or H⁺-ATPase. Results are representative of findings from at least four mice.