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. 2014 Dec 31;8:99–109. doi: 10.2147/OTT.S68765

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© 2015 Liu et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License.

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Effect of CKHE-E on human hepatoma cells intrinsic pathway of apoptosis. (A) HA22T/VGH and HepG2 cells treated with 1.0 mg/mL CKHE-E for 12 and 24 hours. Cells were then harvested and lysed for the detection of cleaved caspase-3, Bax, Bcl-2, and β-actin protein expression. (B) HepG2 cells treated with 0.25 mg/mL CKHE-E for 6, 12, 24, and 48 hours. Cells were then harvested and lysed for the detection of caspase-8 and -9 activity assay. (C) HA22T/VGH and HepG cells were co-incubated with the cell-permeable caspase-3 inhibitors (50 μM) and CKHE-E (1 and 2 mg/mL) for 48 hours, respectively. The effect on cell growth was examined by MTT assay, and the percentage of cell proliferation was calculated by defining the absorption of cells with DMSO as 100%.

Notes: This experiment was repeated three times. Bar represents the SEM. Values were significantly different from the control group. *P<0.05.

Abbreviations: CKHE-E, ethanol Cinnamomum kanehirai Hayata extract; DMSO, dimethyl sulfoxide; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; SEM, standard error of the mean.

Effect of CKHE-E on human hepatoma cells intrinsic pathway of apoptosis. (A) HA22T/VGH and HepG2 cells treated with 1.0 mg/mL CKHE-E for 12 and 24 hours. Cells were then harvested and lysed for the detection of cleaved caspase-3, Bax, Bcl-2, and β-actin protein expression. (B) HepG2 cells treated with 0.25 mg/mL CKHE-E for 6, 12, 24, and 48 hours. Cells were then harvested and lysed for the detection of caspase-8 and -9 activity assay. (C) HA22T/VGH and HepG cells were co-incubated with the cell-permeable caspase-3 inhibitors (50 μM) and CKHE-E (1 and 2 mg/mL) for 48 hours, respectively. The effect on cell growth was examined by MTT assay, and the percentage of cell proliferation was calculated by defining the absorption of cells with DMSO as 100%.

Notes: This experiment was repeated three times. Bar represents the SEM. Values were significantly different from the control group. *P<0.05.

Abbreviations: CKHE-E, ethanol Cinnamomum kanehirai Hayata extract; DMSO, dimethyl sulfoxide; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; SEM, standard error of the mean.