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. 2014 Dec 31;10:283–295. doi: 10.2147/IJN.S74746

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© 2015 Chen et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Confocal microscope images of rMSCs cultured on the representative samples in serum-free growth medium for 3 hours.

Notes: The samples were prepared as follows: 24-hour mineralization (Control); 24-hour mineralization, 24-hour coprecipitation of mineral and OGP (cO); 24-hour mineralization, OGP adsorption (aO); 24-hour mineralization, FN adsorption (aF); 24-hour mineralization, 24-hour coprecipitation of mineral and OGP, FN adsorption (cO/aF); 24-hour mineralization, OGP and FN adsorption (aOF). DAPI stained the nuclei (blue), and rhodamine phalloidin stained the cytoskeleton (red).

Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; OGP, osteogenic growth peptide; FN, fibronectin; rMSCs, rat mesenchymal stem cells.