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. 2014 Dec 31;10:283–295. doi: 10.2147/IJN.S74746

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© 2015 Chen et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Normalized ALP activities with respect to total protein of rMSCs cultured on the representative samples for 3, 7, and 14 days.

Notes: rMSCs were cultured on samples in mineralizing medium as follows: 24-hour mineralization (Control); 24-hour mineralization, 24-hour coprecipitation of mineral and OGP (cO); 24-hour mineralization, OGP adsorption (aO); 24-hour mineralization, FN adsorption (aF); 24-hour mineralization, 24-hour coprecipitation of mineral and OGP, FN adsorption (cO/aF); 24-hour mineralization, OGP and FN adsorption (aOF); or rMSCs were cultured on 24-hour mineralization samples in osteogenic differentiation medium (Positive control). The values represent the mean ± standard deviation (n=4 or 5). In the table, pairwise comparisons of each of the groups were performed using the Student–Newman–Keuls post hoc comparison test, and significance is P<0.05.

Abbreviations: ALP, alkaline phosphatase; OGP, osteogenic growth peptide; FN, fibronectin; rMSCs, rat mesenchymal stem cells.