OC production of rMSCs cultured on the representative samples for 7, 14, and 21 days.
Notes: rMSCs were cultured on samples in mineralizing medium as follows: 24-hour mineralization (Control); 24-hour mineralization, 24-hour coprecipitation of mineral and OGP (cO); 24-hour mineralization, OGP adsorption (aO); 24-hour mineralization, FN adsorption (aF); 24-hour mineralization, 24-hour coprecipitation of mineral and OGP, FN adsorption (cO/aF); 24-hour mineralization, OGP and FN adsorption (aOF); or rMSCs were cultured on 24-hour mineralization samples in osteogenic differentiation medium (Positive control). The values represent the mean ± standard deviation (n=4 or 5). In the table, pairwise comparisons of each of the groups were performed using the Student–Newman–Keuls post hoc comparison test, and significance is P<0.05.
Abbreviations: OC, osteocalcin; OGP, osteogenic growth peptide; FN, fibronectin; rMSCs, rat mesenchymal stem cells.