Larval and adult heart contraction rates. Heart contraction rates were quantified in larvae and adults after receiving no treatment (naïve), a needle wound (injury), an injection with PBS, or an injection with 2 μm fluorescent microspheres in PBS. Across all comparisons, larvae displayed lower heart rates than adults (two-way ANOVA, P<0.0001), but treatment did not affect heart rates (P=0.6725). Whiskers denote the s.e.m.