Fig. 1.
Expression profiles of (a,b) angiotensin-converting enzyme (ACE) and (c,d) angiotensin AT1a receptor genes in hearts from Npr1+/+ and Npr1−/− mice. (a,c) Representative reverse transcription–polymerase chain reaction (RT-PCR) mRNA expression analysis of ACE and AT1a receptor expression in Npr1+/+ and Npr1−/− mice with and without antihypertensive drug treatment. UT, untreated; HYZ, hydralazine; CAP, captopril. (b,d) Densitometry analysis of mRNA transcripts normalized against the expression of GAPDH. The RT-PCR was performed using gene-specific primers. Values are expressed as the mean ± SEM (n = 8/group). ***P < 0.001. Reproduced with permission from Vellaichamy et al.16