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. Author manuscript; available in PMC: 2015 Feb 5.
Published in final edited form as: Circ Res. 2010 Jan 28;106(5):971–980. doi: 10.1161/CIRCRESAHA.109.210682

Figure 1. Experimental design.

Figure 1

a, Culture protocol for CSps, CDCs, and IICSps isolation. b, Two sets of functional experiments were based on CDCs: in vivo injection of 1×105 cells in a SCID mouse MI model for regional tissue samples analysis, histology, and luciferase detection; and in vitro assessment of CDC-CM effects on NRVM viability or HUVEC angiogenic abilities. CMs were collected after 48 hours from CDCs (or NHDFs as control) and used to plate NRVMs in 2% hypoxia or HUVECs on matrix-coated wells.