Table 3.
Effect of steroid by dilution, overall
| Dilution | Steroid | Mean | SD | p value* | Pairwise comparisons† |
|---|---|---|---|---|---|
| 0.00% | Beta | 1.03 | 0.12 | 0.204 | Beta vs Dex (DMOD = 0.035; 95% CI, −0.014–0.085) |
| Dex | 1.00 | 0.07 | Depo vs Beta (DMOD = 0; 95% CI, −0.05–0.05) | ||
| Depo | 1.03 | 0.09 | Beta vs Kenalog (DMOD = 0.009; 95% CI, −0.04–0.059) | ||
| Kenalog | 1.02 | 0.09 | Depo vs Dex (DMOD = 0.036; 95% CI, −0.014–0.085) | ||
| Kenalog vs Dex (DMOD = 0.026; 95% CI, −0.024–0.076) | |||||
| Depo vs Kenalog (DMOD = 0.01; 95% CI, −0.04–0.059) | |||||
| 3.125% | Beta | 0.74 | 0.12 | < 0.0001 | Dex vs Beta (DMOD = 0.123; 95% CI, 0.066–0.179; p < 0.001) |
| Dex | 0.86 | 0.09 | Beta vs Depo (DMOD = 0.001; 95% CI, −0.055–0.057; p > 0.999) | ||
| Depo | 0.74 | 0.08 | Kenalog vs Beta (DMOD = 0.078; 95% CI, 0.022–0.135; p = 0.003) | ||
| Kenalog | 0.82 | 0.08 | Dex vs Depo (DMOD = 0.124; 95% CI, 0.068–0.18; p < 0.001) | ||
| Dex vs Kenalog (DMOD = 0.044; 95% CI, −0.012–0.101; p = 0.17) | |||||
| Kenalog vs Depo (DMOD = 0.08; 95% CI, 0.023–0.136; p = 0.002) | |||||
| 6.25% | Beta | 0.27 | 0.11 | < 0.0001 | Dex vs Beta (DMOD = 0.471; 95% CI, 0.388–0.553; p < 0.001) |
| Dex | 0.74 | 0.14 | Depo vs Beta (DMOD = 0.268; 95% CI, 0.186–0.351; p < 0.001) | ||
| Depo | 0.54 | 0.11 | Kenalog vs Beta (DMOD = 0.37; 95% CI, 0.288–0.453; p < 0.001) | ||
| Kenalog | 0.64 | 0.10 | Dex vs Depo (DMOD = 0.203; 95% CI, 0.12–0.285; p < 0.001) | ||
| Dex vs Kenalog (DMOD = 0.101; 95% CI, 0.018–0.183; p = 0.011) | |||||
| Kenalog vs Depo (DMOD = 0.102; 95% CI, 0.02–0.184; p = 0.01) | |||||
| 12.5% | Beta | 0.02 | 0.03 | < 0.0001 | Dex vs Beta (DMOD = 0.505; 95% CI, 0.427–0.583; p < 0.001) |
| Dex | 0.53 | 0.12 | Depo vs Beta (DMOD = 0.288; 95% CI, 0.21–0.366; p < 0.001) | ||
| Depo | 0.31 | 0.11 | Kenalog vs Beta (DMOD = 0.434; 95% CI, 0.356–0.512; p < 0.001) | ||
| Kenalog | 0.45 | 0.01 | Dex vs Depo (DMOD = 0.217; 95% CI, 0.139–0.295; p < 0.001) | ||
| Dex vs Kenalog (DMOD = 0.071; 95% CI, −0.006–0.149; p = 0.083) | |||||
| Kenalog vs Depo (DMOD = 0.146; 95% CI, 0.068–0.224; p < 0.001) | |||||
| 25.0% | Beta | 0.00 | 0.00 | < 0.0001 | Dex vs Beta (DMOD = 0.302; 95% CI, 0.246–0.359; p < 0.001) |
| Dex | 0.30 | 0.08 | Depo vs Beta (DMOD = 0.122; 95% CI, 0.066–0.179; p < 0.001) | ||
| Depo | 0.12 | 0.06 | Kenalog vs Beta (DMOD = 0.177; 95% CI, 0.121–0.234; p < 0.001) | ||
| Kenalog | 0.18 | 0.11 | Dex vs Depo (DMOD = 0.18; 95% CI, 0.123–0.236; p < 0.001) | ||
| Dex vs Kenalog (DMOD = 0.125; 95% CI, 0.068–0.181; p < 0.001) | |||||
| Kenalog vs Depo (DMOD = 0.055; 95% CI, −0.001–0.112; p = 0.059) | |||||
| 50.0% | Beta | 0.00 | 0.00 | < 0.0001 | Dex vs Beta (DMOD = 0.198; 95% CI, 0.167–0.23; p < 0.001) |
| Dex | 0.20 | 0.06 | Depo vs Beta (DMOD = 0.006; 95% CI, −0.025–0.037; p = 0.953) | ||
| Depo | 0.01 | 0.02 | Kenalog vs Beta (DMOD = 0.032; 95% CI, 0.001–0.063; p = 0.045) | ||
| Kenalog | 0.03 | 0.05 | Dex vs Depo (DMOD = 0.192; 95% CI, 0.161–0.223; p < 0.001) | ||
| Dex vs Kenalog (DMOD = 0.167; 95% CI, 0.135–0.198; p < 0.001) | |||||
| Kenalog vs Depo (DMOD = 0.026; 95% CI, −0.006–0.057; p = 0.145) | |||||
| 75.0% | Beta | 0.00 | 0.00 | < 0.0001 | Dex vs Beta (DMOD = 0.091; 95% CI, 0.066–0.116]; p < 0.001) |
| Dex | 0.09 | 0.06 | Depo vs Beta (DMOD = 0; 95% CI, −0.025–0.025; p > 0.999) | ||
| Depo | 0.00 | 0.00 | Beta vs Kenalog (DMOD = 0; 95% CI, −0.025–0.025; p > 0.999) | ||
| Kenalog | 0.00 | 0.00 | Dex vs Depo (DMOD = 0.091; 95% CI, 0.066–0.116; p < 0.001) | ||
| Dex vs Kenalog (DMOD = 0.091; 95% CI, 0.066–0.116; p < 0.001) | |||||
| Depo vs Kenalog (DMOD = 0; 95% CI, −0.025–0.025; p > 0.999) | |||||
| 100.0% | Beta | 0.00 | 0.00 | < 0.0001 | Dex vs Beta (DMOD = 0.005; 95% CI, 0–0.009; p = 0.024) |
| Dex | 0.00 | 0.01 | Beta vs Depo (DMOD = 0; 95% CI, −0.004–0.004; p > 0.999) | ||
| Depo | 0.00 | 0.00 | Beta vs Kenalog (DMOD = 0; 95% CI, −0.004–0.004; p > 0.999) | ||
| Kenalog | 0.00 | 0.00 | Dex vs Depo (DMOD = 0.005; 95% CI, 0–0.009; p = 0.024) | ||
| Dex vs Kenalog (DMOD = 0.005; 95% CI, 0–0.009; p = 0.024) | |||||
| Depo vs Kenalog (DMOD = 0; 95% CI, −0.004–0.004; p > 0.999) |
Beta = betamethasone; Dex = dexamethasone; Depo = methylprednisolone; Kenalog = triamcinolone; DMOD = difference in mean optical density; *p value of the four-way ANOVA; †pairwise comparisons performed only when the overall ANOVA test was statistically significant (p < 0.05). Pairwise comparison p values were adjusted with the Tukey method to guard against Type I error.