Fig. 1.

Schematic representation of the in vitro co-culture system that was established. Caco-2 cells were grown in 6-well cell culture inserts with a semipermeable support membrane. Murine RAW 264.7 macrophages were cultured in 6-well culture plates. Culture inserts containing Caco-2 cells were placed in the 6-well plates to establish the co-culture system.