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. 2014 Aug 30;9(1):3–10. doi: 10.4162/nrp.2015.9.1.3

Fig. 5.

Fig. 5

SQE, tricin, and P-coumaric acid down-regulate the expression of iNOS and COX-2 in a co-culture system of Caco-2 and RAW 264.7 cells. SQE, tricin (1 µM), and P-coumaric acid (PC, 2.4 µM) were individually added into the apical compartment of the Caco-2/RAW 264.7 co-culture model. After 3 h, 1 µg/ml LPS was added to the basolateral compartment and cells were incubated overnight. Expression of iNOS and COX-2 were then analyzed by western blot. (A) Following the addition of various doses of SQE (100, 200, 400 µg/ml), levels of iNOS and COX-2 were analyzed. The representative blots were shown (left panel) and quantified iNOS (middle panel) and COX-2 (right panel) were shown after normalization to α-tubulin. (B) A equivalent dose of tricin or PC based on 400 µg/ml SQE was added, and levels of iNOS and COX-2 were analyzed. The representative blot has shown (left panel). The quantified iNOS (middle panel) and COX-2 (right panel) were shown after normalization to α-tubulin. One-way ANOVA was applied using Tukey's post-hoc test (α = 0.05). The different letters indicate significant differences. SQE, Sasa quelpaertensis extract; PC, P-coumaric acid, LPS, lipopolysaccharide.