Figure 3. Reduced availability of NSUN5 increases stress resistance of model organisms.

(a) Flies harbouring two individual RNAi constructs of dNsun5 in three lines show increased survival compared with control, when subjected to heat shock (4 h at 37 °C, N=100, two biological replicates, one-way ANOVA with Dunnett’s post test, α=0.05, *P<0.05). Error bars represent s.e.m. (b) nsun-5 mutant worms (JGG1) subjected to 20 mM paraquat treatment for 3 days show significantly increased resistance to the drug (four plates with 30 worms each per genotype, two-sided Student’s t-test, ****P<0.0001). Error bars represent s.e.m. (c) Yeast serial dilution growth assays demonstrate increased resistance of rcm1Δ cells to H₂O₂ (upper panel) and to heat shock (lower panel). Uncropped pictures of the entire plates are given in Supplementary Fig. 5.