Figure 1. UCHL1 upregulates HIF-1 activity.

(a,b) HeLa/5HRE-Luc (a) and MCF7/5HRE-Luc (b) cells were transfected with pcDNA4/UCHL1 (UCHL1) or pcDNA4/myc-His A (empty vector: EV), cultured under normoxic (20%) or hypoxic (1%) conditions for the indicated period, and subjected to the luciferase assay and western blotting using the indicated antibodies. (c) 293T (left) and MDA-MB-436 (right) cells transiently transfected with p5HREp-Luc were treated with scramble-siRNA (Scr) or UCHL1-siRNA (siUCHL1), cultured under normoxic (20%) or hypoxic (1%) conditions for 24 h, and subjected to the luciferase assay. (d,e) HeLa (d) and 293T (e) cells were transiently transfected with either pcDNA4/UCHL1 (UCHL1) or pcDNA4/myc-His A (EV) (d) or treated with either scramble-siRNA (Scr) or UCHL1-siRNA (siUCHL1) (e), cultured under normoxic (20%) or hypoxic (1%) conditions for 24 h, and then subjected to western blotting using the indicated antibodies and qRT–PCR to quantify the mRNA levels of the indicated genes. Mean±s.d. n=3. *P<0.05, **P<0.01, Student’s t-test (a,b,d) and Dunnett’s test (c,e).