Fig. 1.

Flow diagrams of the three employed breeding strategies. In the F₂–DH strategy, DH lines were generated from F₂. In the modified SSD strategy, three seeds from each plant were harvested and bulked from F₃ to F₆ generation. F₂ enrichment was applied in all three strategies to increase the frequency of target genotypes. In the modified SSD strategy, the pedigree method was used in F₂ generation. Seeds from selected plants were bulked after F₂ in the RIL strategy. All seeds were bulked for other generations. The final selection of homozygous target genotype using MAS was conducted in F₂–DH or in F₆ generation of the RIL and modified SSD strategy. Shaded boxes with bold frames stand for seeds of one generation, either bulked (larger boxes) or single harvested by pedigree (narrow boxes in modified SSD) or three seeds bulked per plant (boxes with subdivisions in modified SSD), arrows indicate the production of a new generation. Frames indicate generations where MAS is applied