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Functional characterization of Lg-CRDs. (A) Delayed elution of Lg-CRDs and BSA in Mannan-Agarose affinity column. BSA, used as a negative control (dotted-dashed line), Lg-CRDWT (solid line), Lg-CRDF241L (dotted line), and Lg-CRDW264R (gray dashed line). (B and C) Sensorgrams of the interaction of Lg-CRDs with gp120. B: Lg-CRDWT. C: Lg-CRDF241L. Both proteins were injected in series of twofold dilutions over the concentration range [12.5 μM, 400 μM].
