Table 1.
Structural parameters derived from SAXS data
| Molecule | Rg Guinier (Å)a | Rg P(r) (Å) | Dmax (Å) | c (mg/ml) | MM (kDa) from I(0)b | MM(kDa) from volumec | calculated MM (kDa)d |
|---|---|---|---|---|---|---|---|
| Lg-ECDWT | 55.6 ± 1 | 58.4 ± 1 | 210 | 0.71 | 88.8 | 92.4 | 88.2 |
| Lg-ECDF241L, trimer | 55.9 ± 1 | 55.6 ± 1 | 200 | 0.20 | 92.7 | 87.8 | 88.1 |
| Lg-ECDF241L, dimer | 41.8 ± 1 | 42.9 ± 1 | 140 | 0.49 | 58.9 | 55.6 | 58.7 |
| Lg-ECD model | 65.2e | 69.3 | 230 | 88.2 |
a
Rg obtained with the Guinier approximation in the range 0.4 < qRg < 1.
b
Molar mass M obtained from the I(0) value using the partial specific volume 0.732 cm3.g−1 calculated with the program SEDNTERP available at http://sednterp.unh.edu/.
c
Molar mass M obtained from the whole I(q) curve (qmax = 0.3Å) using the macromolecule volume and the SAXS-MoW method available at http://www.if.sc.usp.br/∼saxs/.
d
The calculated masses were derived from the sequence.
e
The Guinier Rg value is identical to the P(r) derived value when calculated over the experimentally inaccessible q-range q Rg <0.14.