Figure 5.

3D reconstruction of ICDs from failing cells reveals elongated plicae. (A) Plicae (indicated by arrows) are extended compared to control, dashed arrow indicates interplicate region. (B) Removal of a portion of the ICD reveals that interplicate domains also separate the distended projections. Scale bar = 2 μm. Inset, double-headed arrow indicates base diameter of the finger-like projections; surface capped with a white mesh for clarity. Scale bar = 2 μm. (C) The height of the ICD plicae has increased in the failing myocardium, indicated by the separation between the two surfaces delineating the plicate peaks and troughs. Scale bar = 5 μm (1320 plicae analyzed, 6 ICDs). (D) Box and whiskers plot shows increased plicae heights for HF ICDs (P < 0.01). The dividing line is the median and the upper and lower bars the maximum and minimum values. (E) Plot showing that the length of the interplicate domains (nexus) increases in HF ICDs (P < 0.001). To see this figure in color, go online.