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. 2015 Feb 3;108(3):498–507. doi: 10.1016/j.bpj.2014.12.001

Figure 7.

Figure 7

Vacuoles decorate HF ICDs (A) Serial image of a portion of a HF ICD showing details of intercellular vacuoles sandwiched between the two membrane leaflets (arrowheads); box size 2.0 × 1.4 μm. (B) 3D reconstruction of a HF ICD (green) and the associated vacuoles (segmented in range of colors). (C) ICD after rotation around the x axis showing that the vacuoles have formed over both sides of the disc and are not concentrated to any particular region. Scale bar = 2 μm. (D) 3D reconstruction of a plicate domain and intercellular vacuoles. White arrows indicate vacuoles forming at the tip of the plicae and black arrows the vacuoles between the plicae. Length of reconstruction = 10.43 μm. To see this figure in color, go online.