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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: Dev Biol. 2014 Nov 20;398(2):177–192. doi: 10.1016/j.ydbio.2014.11.005

Figure 2. Cells expressing Wnt11-CreER at the onset of gastrulation contribute to the endodermal and endothelial lineages.

Figure 2

Wnt11-CreER mediated recombination and activation of tdTomato expression was induced by a single dose of tamoxifen administration at E6.0, and embryos were harvested and analyzed at E8.5. (A) Epi-fluorescence of tdTomato alone, and (B) merged with brightfield. Saggital (C-F’) and transverse (G-J”) sections of the embryos co-stained with DAPI (nucleus) revealed that tdTomato expressing cells (red) were present along the endoderm from anterior (F and magnified view in F’, red arrows) to the posterior (F, J and magnified view in J’). A small number of tdTomato expressing cells also co-expressed the endothelial marker PECAM (D, H, J and J”) and were present in the dorsal aorta in the embryo proper (green arrow in F; magnified in F’) and as a few clusters in the yolk sac vasculature (green arrow in J; magnified in J”). The dotted line in (B) indicates the plane of sectioning in G to J”. ant NT (anterior neural tube); DA (dorsal aorta); En (endoderm endoderm); Post. en (posterior endoderm); post NT (posterior neural tube); YSV (yolk sac vasculature).