Table1. Summary of the spatio-temporally specific contribution of the Wnt11-CreER expressing cells to the embryonic heart.
Cre activity was transiently induced in Wnt11-CreER; R26R-tdTomato embryos by a single tamoxifen administration at E6.5-10.5, and embryos were harvested and analyzed at E8.5-12.5 to determine the contribution of tdTomato labeled cell in the epicardium and the myocardium and endocardium in the left ventricle (LV), right ventricle (RV), outflow tract (OFT) and atrium (A).
| Tamoxifen administration (Embryonic Day-E) | Dissection (Embryonic Day –E) | Myocardium | Endocardium | Epicardium | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| LV | RV | OFT | A | LV | RV | OFT | A | |||
| 6.5 | 8.5 | - | - | - | - | ++ | ++ | ++ | ++ | - |
| 7.0 | 9.0 | ++ | - | - | - | +++ | +++ | +++ | ++ | - |
| 7.5 | 9.5/10.5 | +++ | - | - | - | +++ | +++ | +++ | ++ | - |
| 8.0 | 9.5 | ++ | ++ | +/- | - | +++ | +++ | +++ | ++ | - |
| 8.5 | 12.5/16.5 | + | +++ | ++ | - | +++ | +++ | +++ | ++ | + |
| 9.0 | 10.5/12.5 | +/- | ++ | +++ | - | +++ | +++ | +++ | ++ | ++ |
| 9.5 | 12.5 | - | + | ++ | - | +++ | +++ | +++ | ++ | +++ |
| 10.5 | 12.5 | - | - | + | - | +++ | +++ | +++ | ++ | +++ |
The (+) and (-) indicate the presence and absence of tdTomato expressing cell in each compartment, respectively, with (+++) indicating highest levels of tdTomato expressing cells present.