Figure 4.

Loss of autophagy restores PE levels in mcd4-174 grown at restrictive temperature. (A) Lipid analysis shows that total PE levels after growth at 37° were reduced in mcd4-174 strains and increased in mcd4-174 atg7∆ and mcd4-174 cho2∆. The total amount of PE was calculated as the sum of all PE species. Data are average and SD of independent biological replicates (n = 3), shown in the “Summary” of Table S2. *P < 0.05, t-test as compared with WT. (B) Growth curves of mutant yeast strains measuring OD₆₀₀ change over time. _.Addition of 5 mM ethanolamine to the medium can partially rescue the growth of mcd4-174 cells at restrictive temperature. The growth of WT (not shown) or mcd4-174 atg7∆ cells was unaffected by the addition of ethanolamine. Data are average and SD of independent biological replicates (n = 3).