Figure 1.

The lost GFP phenotype is worms expressing Aβ₄₂ in ASE neurons. (A) Percent of Aβ₄₂-expressing (FDX25) and control (FDX2512) animals with detectable GFP fluorescence in ASER as a function of age (lost GFP phenotype). n = 4 experiments/genotype. Inset: Representative pictures of ASER neurons of 5- and 12-day-old FDX25 and FDX2512 control worms. (B) Lost GFP phenotype in FDX25 worms in control or subjected to CED-4 RNAi. n = 3 experiments. (C) Lost GFP phenotype in FDX25 worms grown in absence or presence of 1 μg/ml Q-VD-Oph dissolved in the NMG agar media. n = 3 experiments. In a single experiment, 30–50 age-synchronized worms were grown under standard conditions and scored every day for GFP in the ASER neuron. In all figures, mean ±SEM. Error bars smaller than the size of the symbols are not visible in the figure.