Table 1. Additional chromosome III SNP primers.
| Primers set | Sequence (5′ to 3′) | ||
|---|---|---|---|
| S1 | CCAACTGTCGACGATAGAGATG | ||
| AATTCCGTGTAGCGAATCG | |||
| S2 | TTTCAAGACAAGGCAAGTATCC | ||
| TTTGATCAATCAGTGGGTCTTG | |||
| S3 | AATTGCCGAACAGGAAAATG | ||
| CACGCGTGTAACTCTTCCAA | |||
| S4 | CATCGGATACATGGTGCATCG | ||
| GGCAAGTTTTCCTTCTCGTCC | |||
| S5 | TGGTCCCCAGAGATTTCAGA | ||
| GAGTACGCGGGTCATTTTTG | |||
| S6 | GACCGAATCGAATCGGAAG | ||
| AAACAAGGATCAGTGGAATGTG | |||
| S7 | ATGGCCGTGGAATTGATTGC | ||
| ACCCGGAACCCATTTGTAGA | |||
| S8 | GCGGATTCTCGATAAAAACTAC | ||
| AAGCAGAAAAGTGGCATTCA | |||
| S9 | CATCTGAAACTCACGGGAAGTC | ||
| AGTTACAACCTCGGGTCGTG | |||
| S10 | GAATTTTGCTGTGCCAAGCC | ||
| CGCTCACTGTGTCAAAGTGT | |||
| S11 | CATTAGGAAGTGATGCAAGTGG | ||
| TGGATTTGAGAGGTGTCCATAG | |||
| S12 | TCACATCATAAGTCAAGCGGA | ||
| TCGATCTCTAGGCGGATAATCA | |||
| RFLP | N2 digest (bp) | CB4856 digest (bp) | |
| S1 | EcoRI | 262, 205 | 467 |
| S2 | RsaI | 31, 299 | 334 |
| S3 | PsiL | 60, 407 | 467 |
| S4 | XmnI | 442 | 138, 304 |
| S5 | MseI | 419 | 255, 164 |
| S6 | HpyCH4 V | 434 | 244, 190 |
| S7 | RsaI | 423 | 165, 258 |
| S8 | AciI | 463 | 236, 227 |
| S9 | RsaI | 243, 278 | 521 |
| S10 | AclI | 276, 193 | 469 |
| S11 | AvaII | 196, 294 | 490 |
| S12 | HpyCH4 III | 344 | 237, 107 |
SNP primers sequences. The forward primer is listed first; restriction enzymes and band sizes are indicated.