Figure 3.

The sax-7gtl-2 Ric phenotype can be suppressed with nonneuronal gtl-2 expression and culturing of mutant animals on media with reduced Mg²⁺ levels. (A) Expression of wild-type gtl-2 driven in hyp7 or in the excretory cell by the dpy-7 and exc-5 promoters, respectively, can suppress the sax-7gtl-2 Aldicarb resistance just as well as the gtl-2 gene. In contrast, gtl-2 expressed in neurons or muscle by the unc-119 and myo-3 promoters, respectively, cannot suppress the sax-7gtl-2 Ric phenotype. Two to three independent transgenic lines were analyzed for each construct assessed. The rescue abilities of these constructs were similar in all sax-7gtl-2 backgrounds but only data for the sax-7(eq1) gtl-2(tm1463) background are shown. (B) The Ric phenotype exhibited by sax-7gtl-2 animals cultured on standard NGM, which is supplemented with 1 mM Mg²⁺, is dramatically suppressed when animals are cultured on NGM lacking Mg²⁺ supplementation. Error bars show the standard error of the mean of at least 12 sample sets, where n = 25 for each set.