Figure 1.

comatose mutants exhibit shortened lifespan and neurodegeneration. (A and B) Lifespan of comt mutants at 29° (A) and at room temperature (B). (C–E) Histological frontal sections of control (C), and comt (D and E) mutant brains before or after 48-hr exposure to 29°. Neurodegeneration is characterized by vacuolar lesions in the neuropil. Insets in C–E are enlarged images of boxed area. (F) Average number of vacuoles per brain hemisphere for each genotype. Bar in E′, 50 μm. (**P < 0.01 and ***P < 0.001, log-rank test.)