Figure 4.
Maximum growth rates of tetrad lines in YPD + 9 mM CuSO4. Tetrads were derived from four different CBM lines: (A) CBM2, (B) CBM14, (C) CBM25, and (D) CBM34. For each line, maximum growth rate was assayed within the Bioscreen C, with bars representing ±1 SE across 3 days. The darkness of the circle represents the line’s relative number of copies of CUP1, as assayed by Southern blot. Presence (+) or absence (−) of a segregating mutation is also noted. The maximum growth rate is shown for all tetrad lines, as well as for their two parents, BY4739 and the relevant CBM parent (red lines), except for the tetrads derived from CBM25 for which parental growth rate was not assayed (due to its initially being considered CBM22, see Materials and Methods).