Table 2. Oligonucleotides employed for RT-PCR, Southern blot analysis, and genotyping in the forward and reverse directions.
Primer name | Sequence | Experiment |
---|---|---|
CUP1-F | AGCTGCAAAAATAATGAACAATGC | RT |
CUP1-R | GCATTTGTCGTCGCTGTTACA | RT |
TAF10-F | AAGTTGTTCTGACGGTGAACGA | RT |
TAF10-R | GCGACCTATATTGAGCCCGTATT | RT |
CUP1-F | 5Biosg/TTAATTAACTTCCAAAATGAAGGTCA | SB |
CUP1-R | 5Biosg/AGACTATTCGTTTCATTTCCCAGAG | SB |
MAM3-F | AATGAGTGCCGATACCATCC | GT |
MAM3-R | GATTCGTCCCAATCTTTTGC | GT |
VTC4-F | GTTCATGATCTAGCAAAGTTTTCG | GT |
VTC4-R | GGTAACCAAAATGGGATTGAA | GT |
LYS2-F | TCAAGGGCTGAAAAGACAATCAA | GT |
LYS2-R | CGACGCAAAGAGATGAAACCA | GT |
RT, real-time PCR; SB, Southern blot; GT, genotyping; F, forward; R, reverse.