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. 2014 Dec 17;199(2):555–571. doi: 10.1534/genetics.114.171124

Table 2. Oligonucleotides employed for RT-PCR, Southern blot analysis, and genotyping in the forward and reverse directions.

Primer name Sequence Experiment
CUP1-F AGCTGCAAAAATAATGAACAATGC RT
CUP1-R GCATTTGTCGTCGCTGTTACA RT
TAF10-F AAGTTGTTCTGACGGTGAACGA RT
TAF10-R GCGACCTATATTGAGCCCGTATT RT
CUP1-F 5Biosg/TTAATTAACTTCCAAAATGAAGGTCA SB
CUP1-R 5Biosg/AGACTATTCGTTTCATTTCCCAGAG SB
MAM3-F AATGAGTGCCGATACCATCC GT
MAM3-R GATTCGTCCCAATCTTTTGC GT
VTC4-F GTTCATGATCTAGCAAAGTTTTCG GT
VTC4-R GGTAACCAAAATGGGATTGAA GT
LYS2-F TCAAGGGCTGAAAAGACAATCAA GT
LYS2-R CGACGCAAAGAGATGAAACCA GT

RT, real-time PCR; SB, Southern blot; GT, genotyping; F, forward; R, reverse.