Figure 2.

Ranked protein abundance rProt (protein abundance is measured by GFP normalized fluorescence; data kindly provided by Dr. Plotkin) increases with translation elongation efficiency I_TE, except for the group with extraordinarily strong secondary structure at the 5′ end (the MFE1 group). rProt also increases with decreasing stability of secondary structure, with MFE1 having the most stable and MFE4 the weakest secondary structure. The range of MFE is indicated for each of the four MFE groups.