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. 2015 Feb 5;5:8269. doi: 10.1038/srep08269

Figure 5. Decreased apoptotic cell death and demyelination in the injured spinal cord of Crmp4−/−mice.

Figure 5

(a) Representative images of sagittal sections of TUNEL-positive apoptotic cells at 1 week post SCI. (a′) Magnified images of the indicated areas in a. (b) Quantitative analysis of the numbers of TUNEL-positive apoptotic cells showed decreased apoptotic cells in Crmp4−/− spinal cords when compared with those in Crmp4+/+ controls (*, P < 0.05, **, P < 0.01, ***, P < 0.001). Statistical analysis was performed using an unpaired Student's t test. n = 5 mice for each genotypes. Data are mean ± S.E.M. (c) Nissl staining in cross sections of the center and 1.5 mm caudal to injury site revealed increased cell survival in Crmp4−/− spinal cords when compared with in Crmp4+/+ controls at 4 weeks post SCI. (d) Images from cross sections of the center and 900 μm caudal to injury site with myelin staining. Crmp4−/− spinal cords showed higher density of myelin signals and larger are of white matter at both positions when compared with in Crmp4+/+ controls at 4 weeks post SCI. Scale bar: 100 μm in a,a′,d′, 500 μm in c,d.