Figure 7. Cyclic Lac or LacPG-mediated decolourisation of AO7 solution: (a) decolourisation efficiency for active or deactivated biocatalysts (deactivated at 60°C for at least 24 h) and residual enzymatic activity and (b) fresh AO7 solution (100 mg·L⁻¹) and effluents from the 10th cyclic run.

Biodegradation of 100 mg·L⁻¹ AO7 phosphate buffer solution (pH 5.0, 0.05 M) containing 0.1 mM HBT and LacPG or native laccase (0.1 mg laccase protein per mL, ca. 0.8 U·mL⁻¹) at 25°C; LacPG or Lac were recycled for continuous runs; ultrafiltration: pressure = 0.1 MPa, 25°C; error bars show standard deviations for triplicate measurements.