Table 1. Michaelis-Menten kinetic parameters of Lac and LacPG using ABTS and DMP as substrates (pH 5.0, 25°C); error bars show standard deviations from triplicate measurements.

	ABTS			DMP
Enzyme	kcat (s−1)	Km(μM)	kcat/Km (s−1 mM−1)	kcat (s−1)	Km(μM)	kcat/Km (s−1 mM−1)
Lac	5.2 ± 0.2	23.3 ± 3.4	222.8 ± 6.7	2.8 ± 0.5	46.1 ± 4.6	60.0 ± 3.8
LacPG	5.0 ± 0.4	56.0 ± 5.6	89.8 ± 4.5	2.5 ± 0.9	74.6 ± 8.0	33.6 ± 1.4