Figure 4.
EMMPRIN down-regulation by scFv-M6-1B9 intrabody affecting decline of CEA level. Caco-2 cells transduced for 7 days and 10 days were collected as the supernatant, extracted cells for total RNA, and reverse transcribed into cDNA. (A) CEA secretions in the Caco-2 cell supernatant. The amount of CEA released into the medium was determined using an automated analyzer. (B) The expression of CEA mRNA was analyzed using real-time RT-PCR. GAPDH mRNA was used as the internal control. The normalized mRNA expression was analyzed using the comparative CT method. The white and black bars represent day 7 and day 10 of incubation, respectively. The data are presented as mean±s.e.m. *p<0.05, as determined by one-way ANOVA.