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. 2014 Dec 15;106(1):18–24. doi: 10.1111/cas.12570

Figure 5.

Figure 5

Low-density lipoprotein (LDL) was targeted to the dynamic early endosome followed by translocation to the late endosome/lysosome for degradation. (a) Human monocyte-derived dendritic cells were incubated at 37°C with Alexa 594-labeled LDL. Organelles were stained with an anti-EEA1 mAb, anti-Rab5 polyclonal antibody, and anti-LAMP-1 polyclonal antibody, followed by Alexa 488-conjugated goat anti-rabbit IgG or anti-mouse IgG. Arrowheads indicate colocalization of internalized LDL and each organelle. (b) To quantify the percentage of colocalization, a single z-plane of one cell was evaluated. For each protein and organelle combination, a total of 150 cells (50 cells from three independent experiments) were analyzed. Data are shown as means + SEM of three independent experiments. *P < 0.01.