Table 1.

Quantitation of survivin-2B-specific CD8⁺ T cells by tetramer assay

Patient no.	Tumor	Survivin-2B80-88-specific CD8+ T cell frequency (tetramer staining)						Effect
		In vitro stimulation	(−)	Survivin-2B80-88	Hsp90	Survivin-2B75-93	Hsp90–survivin-2B75-93
1	Colon		0.06	4.87	4.47	3.24	8.47	††
2	Colon		0.32	2.87	0.77	4.01	1.30	No
3	Pancreas		0.70	6.27	1.70	1.88	6.64	††
4	Pancreas		0.48	4.56	0.84	1.28	3.02	†
5	Ampulla of Vater		1.27	4.60	0.97	2.24	6.50	††
6	Breast		3.59	3.78	3.00	3.06	3.82	††
7	Breast		3.98	3.91	1.94	2.28	6.19	††
8	Breast		2.76	3.92	2.91	2.08	6.07	††

^†Frequency of survivin-2B-specific CD8⁺ T cells stimulated with heat shock protein 90 (Hsp90)–survivin-2B_75-93 peptide complex was increased compared with stimulation with survivin-2B_75-93 precursor peptide.

^††Frequency of survivin-2B-specific CD8⁺ T cells stimulated with Hsp90–survivin-2B_75-93 peptide was increased compared with stimulation with both survivin-2B_80-88 peptide and survivin-2B_75-93 peptide. (−), negative control; No, no effect.