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Decrease of mitochondrial membrane potential (Δψm) in cancer-tissue originated spheroids (CTOS) after exposure to high-dose mitomycin C (MMC). (a, b) MitoTracker Red CMXRos (red), Δψm-dependent dye, was evaluated by fluorescence microscopy (a) and flow cytometry (b) in CTOS after 2 h exposure to control medium or MMC. (c) MitoDeepRed (red), Δψm-independent dye, depicts morphology of mitochondria in CTOS after 2 h exposure to control medium or MMC (1 mg/mL).
