Table 1.
Antibody sources, clones, and dilutions
| Antibody | Clone | Dilution | Vendeor |
|---|---|---|---|
| LC3B | 5F10 | 2.5 μg/mL (1:40) | Nanotools; Teningen, Germany |
| Beclin-1 | EPR1733Y | 1:50 | Abcam; Cambridge, UK |
| p62/SQSTM1 | Polyclonal | 2 μg/mL | Sigma-Aldrich; St. Louis, MO, USA |
| CD1a | O10 | Ready-to-use | Beckman Coulter; Brea, CA, USA |
| CD3 | Polyclonal | Ready-to-use | Dako; Glostrup, Denmark |
| CD56 | 123.C3.D5 | Ready-to-use | Thermo Scientific; Waltham, MA, USA |
| HLA class I heavy chain | HC-10/HC-A2 | 0.5 μg/mL each | * |
| HLA class II | LGII-612.14 | 1 μg/mL | * |
| p53 | DO7 | 1:50 | Dako |
| Ki-67 | MIB-1 | 1:300 | Dianova; Hamburg, Germany |
| Negative control | 11711 | 2.5 μg/mL | R&D Systems; Minneapolis, MN, USA |
*
Kind gifts from Dr. Soldano Ferrone, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.