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. 2014 Dec 23;106(1):78–85. doi: 10.1111/cas.12574

Figure 3.

Figure 3

Radiation-activated SRC transduces intracellular signaling of phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) and p38 MAPK to increase migratory and invasive behavior. (a) PI3 kinase assay and Western blot analysis for phosphorylation status of AKT and p38 MAPK in MCF7 cancer cells transfected with siRNA targeting SRC or scrambled control siRNA (si-Cont) prior to irradiation. (b) Western blot analysis for phosphorylation status of AKT in MCF7 cancer cells transfected by dominant negative (DN)-p38 or control pCMV5 prior to irradiation. (c) Migration and invasion assay in MCF7 breast cancer cells transfected with siRNA targeting AKT (si-AKT) or scrambled control siRNA prior to irradiation. β-actin was used as a loading control. Error bars represent mean ± SD of triplicate samples. **P < 0.01. PTEN, phosphatase and tensin homolog. Cont, control.