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. 2014 Mar 17;105(4):445–454. doi: 10.1111/cas.12364

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Fig. 9 — Sirtuin 1 (SIRT1) expression was evaluated in pancreatic cancer samples before and after chemotherapy. (a) SIRT1 expression in pancreatic cancer samples before chemotherapy (BC) and after chemotherapy (AC) (n = 8) was analyzed by immunohistochemical staining with an anti-SIRT1 antibody in paraffin-embedded tissue sections. (b) Representative expression of SIRT1 was analyzed in pancreatic cancer samples before (n = 4) and after chemotherapy (n = 4) by Western blot analysis. The β-actin level was assessed as a loading control. (c) Expression of SIRT1 was also detected in RNA levels by quantitative RT-PCR. Representative results are shown.